Improve the yielding of glucose
Improve the effect of saccharification
Shorten the time
Reduce the dosage of the glucoamylase
PU1L is a liquid-type pullulanase preparation made from excellent
strain of Bacillus Licheniformis through deep fermentation and
extraction technique. Pullulanase is one type of isoamylase which
can selectively hydrolyze pullulan, amylase and corresponding α-1,
6-glucosidic in oligosaccharide, thereby, cutting down the whole
hydrolysis branch enzyme. Being used with other enzymes
(glucoamylase, beta-amylase) can reach the effect of quickening the
reaction speed and improving the content of glucose and maltose. It
is applied broadly in the industries of starch sugar, organic acid,
beer saccharification, modified starch, food, and brewing
Grade: food grade.
Volume weight (Liquid type)
:Normal fermentation odour
:Soluble with little subsidence
Temperature: 40℃~65℃, optimum at 60;
pH: 4.0~6.5, optimum 4.2-4.6.
The amount of pullulanase needed to hydrolyze the pullulan to
produce 1mg reducing sugar (calculated by glucose) at 60°C and
pH4.5 is defined as one enzyme activity unit and marked with u/g
--Starch sugar, glucose maltose, high maltose syrup, high-fructose
--Monosodium glutamate, vinegar and soy sauce.
--In glucose industry, pullulanase and glucoamylase can be used
together in saccharification step. It can improve the yielding of
glucose by reducing the content of oligosaccharides and reduce the
dosage of the glucoamylase;
--In maltose industry, pullulanase and fungal alpha-amylase are
used for saccharification. The pullulanase can improve the yield of
maltose and reduce the content of branched chain oligosaccharides.
It can be used to produce maltose syrup with 70% maltose;
--In beer industry, pullulanase can reduce the content of dextrin
in the manufacture of dry beer. During saccharification, the use of
pullulanase can not only improve the effect of saccharification but
also shorten the time;
--Pullulanase can also be used to improve the content of glucose
and shorten saccharification time in the manufacture of monosodium
glutamate, alcohol, vinegar and soy sauce and so on.
The following dosage is an example of 1,000u/ml. Other
specification can be converted according to the enzyme activity.
--In the manufacture of high maltose syrup (>70%): using
pullulanase with β- amylase in saccharification stage. The
recommended method: adding pullulanase in proportion of 0.5-1.0L/t
of dry basis and β-amylase (based on 700,000u/mL) 0.5-1.0L/t of dry
basis, for 24 hours;
--In the manufacture of super high maltose syrup (>90%): in the
saccharification stage it needs to used with β-amylase. The
recommended method: adding β-amylase in proportion of 1.5-2.0L/t of
dry basis (based on 700,000u/mL), pullulanase 1.5-2.8L/t of dry
basis, saccharifying for 24 hours and adding maltotriose enzyme
1.5-2.0L/t of dry basis, saccharifying for another 24 hours;
--In process of beer brewing: add pullulanase in proportion of
10-50mL/t of wheat juice in saccharifying and fermentation stage,
to improve the fermentation degree and shorten saccharifying time;
--In manufacture of modified starch food: the recommended dosage is
0.6-2.0 L/ton of raw materials;
--In saccharifying process of monosodium glutamate, alcohol,
vinegar, soybean sauce and others: adding pullulanase can reduce
dextrin residue and improve the utilization of raw material. The
recommended dosage is 0.1-0.3L/t of raw material.
PACKAGING & DELIVERY:
25kgs/drum or 1125kgs/drum. Or as per requirement.
This product is an active biological preparation. It should be
stored and transported in a cool, dry and ventilated place avoiding
Under favorable storage condition, the shelf life is 12 months.
Notice: tighten the lid after each usage to avoid pollution or
Enzyme preparations belong to protein, which may induce
sensitization and cause allergic type reactions in sensitized
individuals. Prolonged contact may cause minor irritation for skin,
eyes or mucous membrane of nose, so any direct contiguity with
human body should be avoided. If irritation or allergic response
for skin or eyes develops, consult a doctor.